Fatty acid hydroperoxides biotransformation by potato tuber cell-free extracts
نویسندگان
چکیده
1 Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques, 2, Passage des Déportés, B-5030 Gembloux, Belgium 2 Département de Génie rural, Centre de recherches agronomiques, 146, Chaussée de Namur, 5030 Gembloux, Belgium 3 Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Chaussée de Wavre 1850, B-1160 Bruxelles, Belgium 4 Unité de Biologie Végétale, Faculté Universitaire des Sciences Agronomiques, 2, Passage des Déportés, B-5030 Gembloux, Belgium
منابع مشابه
Potato tubers exhibit both homolytic and heterolytic hydroperoxide fatty acid-cleaving activities.
The action of a crude potato-tuber extract on 9- and 13-hydroperoxides of linoleic and linolenic acids was investigated. HPLC analysis revealed that 50% of the 9-hydroperoxide isomers and almost all the 13-hydroperoxide isomers were rapidly enzymically metabolized. No degradation of fatty acid hydroperoxides was observed with a thermally denatured enzymic extract. GC-MS identification of the vo...
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Plant lipoxygenases (LOXs) are a functionally diverse class of dioxygenases implicated in physiological processes such as growth, senescence, and stress-related responses. LOXs incorporate oxygen into their fatty acid substrates and produce hydroperoxide fatty acids that are precursors of jasmonic acid and related compounds. Here, we report the involvement of the tuber-associated LOXs, designat...
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A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
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The infectious ribonucleic acid (RNA) of potato spindle tuber virus (PSTV) can be separated by hydroxyapatite chromatography from double-stranded RNA detectable in low amounts in both infected and uninfected plant tissue extracts. The chromatographic behavior of ribonuclease-sensitive PSTV RNA resembles that of transfer RNA.
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